| 质粒名称: | pcDNA3/HA-FLAG RBP2 H483A |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 14801 |
| 存储实验室: | William Kaelin |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5446 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Retinoblastoma Binding Protein 2,RBP2,JARID1A,KDM5A |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4900 bp |
| 基因突变: | Changed His 483 to Ala, R46W |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: |
HA（骨干网上的N个终端）
标志（主干上的N个终端）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pCAAT |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3/HA-FLAG RBP2 H483A was a gift from William Kaelin (Addgene plasmid # 14801 ; http://n2t.net/addgene:14801 ; RRID:Addgene_14801) |
| 参考文献部分: | The retinoblastoma binding protein RBP2 is an H3K4 demethylase. Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P, Gilliland DG, Zhang Y, Kaelin WG. Cell. 2007 Mar 9. 128(5):889-900. 10.1016/j.cell.2007.02.013 PubMed 17320163 |
买家导航
pcDNA3/HA-FLAG RBP2 H483A载体质粒基本信息
pcDNA3/HA-FLAG RBP2 H483A质粒图谱载体图谱和pcDNA3/HA-FLAG RBP2 H483A载体序列质粒序列多克隆位点信息
pcDNA3/HA-FLAG RBP2 H483A质粒载体简介
pcDNA3/HA-FLAG RBP2 H483A质粒序列载体序列
LOCUS pcDNA3/HA-FLAG RBP2 H483A 11773 bp ds-DNA circular SYN 29-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pcDNA3/HA-FLAG RBP2 H483A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11773)
AUTHORS Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P,
Gilliland DG, Zhang Y, Kaelin WG
TITLE The retinoblastoma binding protein RBP2 is an H3K4 demethylase.
JOURNAL Cell. 2007 Mar 9. 128(5):889-900.
PUBMED 17320163
REFERENCE 2 (bases 1 to 11773)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 29, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11773
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 907..933
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 952..975
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
promoter complement(7326..7344)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(7327..7344)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(7364..7381)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7370..7594
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7640..8068
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7727..7746)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7937..7958
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8077..8097)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8082..8411
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8262..8397
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8324..8343
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 8478..9272
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8532..8551)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9142..9161
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 9446..9567
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9483..9502)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9537..9556
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9616..9632)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9616..9632)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9629..9651)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9640..9656
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9664..9694)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9709..9730
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9847..9864)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10018..10606)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10098..10117)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10777..11637)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11400..11419
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11638..11742)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 atggcgtacc catacgatgt tccagattac gccaggatcc atcgaggagg agactacaag
961 gacgacgatg acaagggagg acgatgtcga cgaattctgc agatggggat ccgtcgacgg
1021 atggcgggcg tggggccggg gggctacgcg gcggagttcg tgccaccgcc agagtgcccc
1081 gtctttgagc cgagttggga ggagttcaca gatccgctca gctttatcgg ccgcatctgg
1141 cctttggcgg agaaaaccgg catctgcaaa attcggccgc ccaaggactg gcagcctcca
1201 tttgcctgtg aagtaaaaag ctttcgtttc actccaagag tccagcgcct gaatgaactt
1261 gaggcaatga ccagagtgag attggatttc ttggatcaac tagcaaaatt ttgggaactt
1321 caaggatcta ctctgaagat ccctgtggta gagagaaaaa tcctggatct gtatgctttg
1381 agcaagattg ttgccagcaa aggaggtttt gaaatggtca ccaaagagaa gaaatggtct
1441 aaagtgggta gtcgcttggg atatctgcca ggaaaaggaa ctgggtctct tttgaagtca
1501 cattatgaaa gaattctcta cccatatgag cttttccagt ctggtgtgag ccttatgggt
1561 gtgcagatgc ctaatttaga tcttaaagaa aaagtggagc ctgaggttct tagcactgat
1621 acccaaactt ccccagagcc aggcacaagg atgaacattc tgccgaagag aacaagacgt
1681 gtgaagactc agtcagaatc tggagatgtg agtagaaaca cggaactgaa gaaacttcag
1741 atttttgggg ctgggcccaa ggttgtgggc ttggcaatgg gaacaaaaga taaagaagat
1801 gaggtcaccc gaagacgaaa agttaccaac aggtcagacg catttaacat gcaaatgaga
1861 caacggaaag gcactctctc tgttaacttt gttgatctct atgtttgtat gttttgtggt
1921 cggggaaaca atgaagataa attgcttttg tgtgatggat gtgatgacag ctatcataca
1981 ttttgtctaa ttcctccact acctgatgtg cccaaaggag actggaggtg tcctaaatgt
2041 gtcgccgagg aatgtagcaa acctcgagaa gcctttggat ttgaacaagc tgtacgagag
2101 tatacacttc agagctttgg agagatggca gataatttta agtctgatta ttttaatatg
2161 ccagtccata tggttcccac agaactagta gaaaaggaat tttggcggct ggtaagcagc
2221 attgaagaag atgttattgt ggaatatgga gcagatatct cctcaaaaga ctttggaagt
2281 ggatttccgg tgaaggatgg gcggagaaag attctgccag aagaagagga atatgcactt
2341 tctggttgga atttgaataa catgcctgtc ctggaacagt ctgttcttgc acatattaat
2401 gtggacatct ctggtatgaa agtgccgtgg ctctatgtgg gaatgtgctt ctcttctttt
2461 tgctgggcca ttgaggatca ctggagttat tccatcaact acttgcactg gggggagcca
2521 aagacatggt atggtgtgcc atctcatgct gcagagcaac tggaggaggt gatgagagag
2581 ctggcccccg agttatttga atcccagcct gatcttctgc atcagttagt taccatcatg
2641 aaccccaacg tgctaatgga gcatggtgtg cctgtgtaca ggaccaatca gtgtgctggc
2701 gagtttgttg tgacatttcc tcgtgcctat cactctggat ttaaccaggg ctacaacttt
2761 gctgaagctg tgaacttctg tactgctgac tggttgccca ttggacgtca atgtgtaaat
2821 cattaccgac gcctaaggcg ccactgtgtc ttttcacacg aggaactaat tttcaagatg
2881 gcagcagatc cagaatgctt agatgtgggg ctggctgcca tggtctgcaa agaattgact
2941 ctcatgactg aagaagaaac acgattaaga gagtctgttg tacagatggg tgtcctgatg
3001 tcagaagaag aagtgtttga acttgttcct gatgatgagc ggcagtgttc agcatgcaga
3061 accacatgtt ttctctctgc tctcacatgt tcctgtaatc ctgagcggct tgtatgtctc
3121 taccatccaa ctgatctgtg cccctgcccc atgcagaaga aatgtcttag atatcgctac
3181 ccattagaag acctcccttc tctgctatat ggtgtaaaag tcagggcaca gtcctatgac
3241 acttgggtca gtcgtgttac agaagcattg tctgctaact tcaaccacaa aaaagatttg
3301 attgaattgc gagtaatgct ggaagatgct gaggatagga aatacccaga gaatgatctc
3361 tttcgaaaac tcagggatgc tgtaaaagaa gctgagacct gtgcttctgt ggctcagctg
3421 cttctgagca aaaagcagaa acacagacag agcccagata gtgggaggac tcggaccaaa
3481 ctgacagtgg aagaattgaa ggcctttgtc caacaacttt ttagtcttcc gtgtgtcatc
3541 agccaagctc ggcaagtaaa gaatctgcta gatgatgtgg aagagtttca tgaacgtgct
3601 caggaggcca tgatggatga aaccccagat tcttccaaac tccagatgtt gatagatatg
3661 ggctctagtc tctatgtgga actccctgaa ttaccacgac tgaagcaaga gctacaacag
3721 gctcggtggt tggacgaagt aagactgacc ttatcagatc cgcaacaagt cactttggat
3781 gtcatgaaga agctgataga ctctggggta gggttggcac cccaccatgc tgtggagaaa
3841 gcaatggctg aactacagga gctccttaca gtctctgaac gatgggaaga aaaggctaag
3901 gtctgcctac aggcaagacc gaggcacagt gtggcaagtt tagaaagcat tgtgaatgaa
3961 gccaagaaca ttccagcctt tctacccaat gtgttgtcct tgaaagaagc cttacaaaag
4021 gctcgagaat ggaccgctaa agtggaagct attcagagtg gcagcaatta cgcttatttg
4081 gagcagcttg agagcttgtc tgcgaaagga cgccctattc ctgtgcgtct tgaagcactg
4141 ccgcaagtgg aatcacaggt agcagcagca cgggcatgga gagaacggac tgggcggacg
4201 tttcttaaga agaattctag ccatacattg ttacaggtgc tgagcccccg gaccgacatt
4261 ggtgtatatg ggagtggcaa aaataggagg aaaaaagtaa aagaactaat agaaaaagaa
4321 aaagaaaagg atctggacct ggagcctctg agtgatctgg aggaaggatt ggaggaaacc
4381 agagatacag ccatggtggt ggcagttttc aaagaacggg agcaaaaaga gattgaagcc
4441 atgcattctc tcagagcagc caacctagcc aagatgacaa tggtggaccg catagaagaa
4501 gtaaaatttt gcatttgccg caagacagcc agtgggttta tgctacagtg tgagctctgc
4561 aaagactggt tccataacag ctgtgttcct cttcctaaat caagttccca aaaaaaagga
4621 tccagctggc aagctaaaga agtaaaattc ctttgccctc tttgtatgcg gtctcgaagg
4681 cccaggctag agactattct gtcactcctg gtatcccttc agaagttgcc cgtacggttg
4741 cctgaaggag aggccctgca gtgtttgaca gaacgtgcta tgagttggca agatagagcg
4801 cggcaggctc tagccacaga tgaactatcc tctgccctgg ccaaactatc tgtgttgagc
4861 cagcgtatgg tggaacaggc ggctcgagaa aaaactgaaa agatcatcag tgcagaactc
4921 caaaaagcag ctgccaatcc agacttacag ggacacttac ctagtttcca gcagtctgct
4981 tttaaccggg tggtgagcag tgtgtcatct tctcctcgac aaacaatgga ctatgatgat
5041 gaagaaacag actctgatga agacattcga gagacatatg gctacgacat gaaggacaca
5101 gccagtgtga agtcctctag tagtcttgaa cccaatcttt tttgtgatga agagattccc
5161 atcaaatccg aggaggtggt gacccacatg tggacagcac cttcattttg tgcagagcat
5221 gcttattctt ctgcttctaa gagttgttct caaggttcta gcaccccaag gaaacaacct
5281 cggaagagcc ctttggtgcc ccgaagtttg gaacctccag tgctggagtt gtcacctgga
5341 gctaaggcac aactggaaga acttatgatg gttggagatc tcctggaagt atctctggac
5401 gagactcaac acatatggcg gattttgcag gccacacacc caccctctga agacagattc
5461 ttgcatatca tggaggatga cagcatggaa gagaaaccac taaaagtgaa aggaaaggac
5521 tcttcagaga agaaacggaa acggaagcta gaaaaggtag agcaactttt tggagaagga
5581 aaacagaagt ccaaggagtt aaagaaaatg gacaaaccta gaaagaagaa attaaaatta
5641 ggtgcagaca aatcaaagga gctgaataaa ctggccaaga aactagcaaa agaagaagag
5701 agaaagaaaa agaaggagaa ggctgctgca gccaaagttg aacttgtgaa agagagcact
5761 gaaaagaaaa gagagaaaaa ggtgctggac atcccctcaa agtatgactg gtcaggagca
5821 gaggagtctg atgatgagaa tgctgtgtgc gcagcacaga actgccaaag gccctgcaag
5881 gacaagggag ttgtatttgt aacggaagaa gagaagaata aaaaatatta gttttaaaag
5941 tggcctatgt gactgctttt ctaaaaaggt agactgggta caatgtgatg gtggctgtga
6001 tgagtggttt catcaagttt gtgtgggtgt atctccagaa atggctgaaa atgaagatta
6061 catctgtata aactgtgcaa agaagcaggg gccagttagc ccaggtccag caccacctcc
6121 ttccttcata atgagctaca aactaccaat ggaggatctt aaagagacca gttagcagat
6181 gcttggttag tttgggacat ggggggacat ggaccacatt gagaccttag tcatcaagta
6241 gagtggttta tatccacttg gaatgttgct tccaaagatg aatggccttc agagaaagtc
6301 cccttagtgc tggcttcctc tttgcatgga ctctgtgggt tacattctct atcaacatat
6361 ctatgcagag ggtgtcttct ttggtacaac agccaatatt tcatgtctcc tttgagtgtg
6421 gtttactgca ttaaggccag atgcttaatt gagctctagg gtggctggtt agtattaata
6481 cattggtgtg ctaacagggc atataggatg tggcttttgt ccagctgata gtagttagag
6541 gcttacaact taggagcagc accaactgaa ggtgctaatt gcttggatct ccttcattgg
6601 gatagttgga gagggattgg agtaccactt tccttccact gttacctggt acttaatgcc
6661 ctaaagatac aactaggagt aacagggcca aagttatttc tgttagacgt caaggaatgg
6721 tatcacagtc tattgacctc agcgatttgt gcttgtttgt gctagaagaa catcccaaat
6781 aggagaacct ctcacaagct ggggcaggtc accttatctt tgtaagatga ggatatcatc
6841 tagatcagaa atctgactag attggattct gaggagaaga acctactaca aggcaaggag
6901 ccgttttttg gctttgaaaa gtcttgctgt cttgggtcta cattttaggg aagagcaggt
6961 acatggatcc aggcttctgc caaaaaaaaa aagagaagaa gatgacgatg atgaccagtc
7021 gtactatctt actgagccac agtgatgcat gcttttcggg gaaaacttca ttcacaagta
7081 ttccagacac caggcttcag gcatggccat gagcaagacc agcaaataac agctttttgc
7141 cttgcagccc tgaccccaat gtctgctgtt tccaacactg gtgatttcta actacggccc
7201 acagcagatg ctgttgaata acaccatggc ttcatcagag gatgtggggt tgtagtacct
7261 ctgggtgatg aagttgtttt agtaaatcca tttttaaaaa aaaaaaaaaa tctagagggc
7321 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
7381 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
7441 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
7501 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
7561 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
7621 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
7681 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
7741 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
7801 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
7861 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
7921 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
7981 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
8041 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
8101 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
8161 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
8221 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
8281 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
8341 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
8401 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
8461 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
8521 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
8581 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
8641 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
8701 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
8761 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
8821 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
8881 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
8941 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
9001 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
9061 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
9121 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
9181 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
9241 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
9301 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
9361 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
9421 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
9481 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
9541 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
9601 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
9661 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
9721 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
9781 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
9841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
9901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
9961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
10021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
10081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
10141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
10201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
10261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
10321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
10381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
10441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
10501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
10561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
10621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
10681 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
10741 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
10801 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
10861 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
10921 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
10981 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
11041 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
11101 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
11161 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
11221 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
11281 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
11341 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
11401 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
11461 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
11521 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
11581 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
11641 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
11701 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
11761 gccacctgac gtc
//
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